The 7th International Conference on Biomedical Engineering and Biotechnology (ICBEB 2018)
October 17th - 20th, 2018, Nanjing, China
• 中文版     • English
Invited Speakers
Dr. R. James Swanson, Professor & Chair, Department of Anatomical Sciences, Liberty University, USA.

Speech Title: Nanosecond Pulsed Electric Field (nsPEF) Causes Instantaneous Cellular Membrane Modulation by Altering Calreticulin Expression

Abstract: Nanosecond Pulsed Electric Field (nsPEF) is a novel cancer ablation technology which applies high pulsed power into ultra short pulses and causes non-thermal bioeffects on cell membrane. It is hypothesized to open nanopores in the lipid layers on cell membrane but the direct morphological evidence is still lack. This study designed a unique cell capture system to record the instantaneous membrane changes in the early stage of cell death. The melanoma B16-F10 cells and Jurkat T leukemia cells were treated by nsPEF (300 ns, at 2 kV/cm, 4 kV/cm and 8 kV/cm). The morphological changes on plasma membranes caused by nsPEF were snapped in liquid nitrogen after the cells in nsPEF treatment system equipped with free falling track and pulsor. Immediately after a single pulse, the pulsed cells plunged into liquid nitrogen by gravity for scanning electron microscopy (SEM). Beside liquid nitrogen the cell culture media was also used to collect pulsed cells for MTT, apoptosis, cell cycle and CRT expression. The study provides the first hand direct SEM proof of near-real-time cell membrane changes duiring nsPEF-caused cancer cell death. The non-thermal nsPEF induces higher expression of cell surface calreticulin (CRT), a key protein adjusting the lipid raft suspension and trafficking, which contribute to a dynamical membrane rafting, pore perforation and cell death.
Keywords: nanosecond pulsed electric field (nsPEF); membrane perforation; fresh freeze; calreticulin pathway

Dr. Lili Chen, Associate Professor/ Medical Physicist, Department of Radiation Oncology, Fox Chase Cancer Center, PA, USA.

Speech Title: MR Guided Pulsed High Intensity Focused Ultrasound Enhancement of Docetaxel Combined with Radiotherapy for Prostate Cancer Treatment

Abstract: The purpose of this study is to evaluate the efficacy of the enhancement of docetaxel by pulsed focused ultrasound (pFUS) in combination with radiotherapy (RT) for treatment of prostate cancer in vivo. LNCaP cells were grown in the prostates of male nude mice. When the tumors reached a designated volume by MRI, tumor bearing mice were randomly divided into 7 groups (n=5): (1) pFUS alone; (2) RT alone; (3) docetaxel alone; (4) docetaxel + pFUS; (5) docetaxel + RT; (6) docetaxel + pFUS + RT; and (7) control. MR guided pFUS treatment was performed using a focused ultrasound treatment system (InSightec ExAblate 2000) with a 1.5T GE MR scanner. Animals were treated once with pFUS, docetaxel, RT or their combinations. Docetaxel was given by i.v. injection at 5 mg/kg before pFUS. RT was given 2 Gy after pFUS. Animals were euthanized 4 weeks after treatment. Tumor volumes were measured on MRI at 1 and 4 weeks post-treatment. Results showed that triple combination therapies of docetaxel, pFUS and RT provided the most significant tumor growth inhibition among all groups, which may have a potential for the treatment of prostate cancer due to an improved therapeutic ratio.
Keywords: MRgFUS, docetaxel, RT, prostate cancer, in vivo

Dr. Mire Zloh, Professor Emeritus, University of Hertfordshire, UK.

Speech Title: In silico approaches to aid rational design of protein therapeutics formulations

Abstract: Development of protein therapeutics formulation relies on the selection of excipients that stabilize protein and/or prevent aggregation. Intermolecular interactions between excipients and proteins can improve their stability or influence protein-protein interactions. Experimental strategies to optimize formulations use force degradation studies and involve screening many combinations of excipients and buffers. More...
Keywords: protein therapeutics, protein stability, protein–excipient interactions, formulation, molecular docking, molecular dynamics

Dr. Neda Zarrin-Khameh, Associate Professor, Deputy Chief of Anatomic Pathology, Ben Taub Hospital; Department of Pathology & Immunology, Baylor College of Medicine, USA

Speech Title: Strongyloides in Cerebrospinal Fluid

Abstract: A 45-year-old Latino man with diabetes mellitus type II and recent episode of pneumonia presented to the emergency room with complaint of rectal pain for a week. He was found to have a perirectal abscess and admitted for debridement. Screening HIV test was positive with a T lymphocyte count of 2, a diagnosis previously unknown to the patient. Throughout his course of admission, his mental status deteriorates. Strongyloides was found on cytologic evaluation of his CSF. Strongyloides involvement of CSF is rare and most of them are seen on postmortem examination. The main risk factor for developing hyperinfection and disseminated disease is immunosuppression; which frequently seen in patients with advanced HIV infection or patients received corticosteroid treatment.
Keywords: CSF, HIV, Strongyloides

Dr. Robert Guidoin, Professor, Department of Surgery, Faculty of Medicine, Université Laval, Canada

Speech Title: Cardiovascular Devices For Implantology: How The 3bs Concept Contributes To The Culture Of Safety

Abstract: The development of myriads of innovative implantable devices during the last decades proved to be mostly physician and industry driven. The clinicians continue to require more and more sophisticated devices to restore various functionalities and allow more and more patients with complex and sometimes life threatening pathologies to be treated. Thus the life of the patients is prolonged and made more comfortable. More...

Dr. Jesús Zurdo, Senior Director of Strategic Innovation, LONZA Pharma & Biotech, UK

Speech Title: Cross-Functional Workflows in Bioprocessing to Maximize Speed in Development and Therapeutic Performance of Novel Biotherapeutics

Abstract: Bioprocessing has experienced a true revolution in the last couple of decades making possible the commercial success of therapeutic molecules that are now common names in the industry. Biotherapeutics are currently manufactured using hosts and processes that are far more productive and robust than ever before, affording a very tight control over product properties that were unthinkable in a not-too-distant past. More...

Dr. Haiyan Zhou, Professor, Hainan Medical College School of Dental Medicine
Director of the Central Laboratory, the First Affiliated Hospital of Hainan Medical College, China

Dr. William Lafayette Mondy, Department of Pharmaceutical Sciences, College of Pharmacy, University of South Florida, USA

Speech Title: Chroma Code Manufacturing: A High-Resolution Coordination of Tools for Producing Composite Tissues

Abstract: Our goal is to coordinate within a Chroma (color) coded production scheme, a computer-driven system of tools that manages and promote the regeneration of human tissues on a large composite scale. We have two strategies for producing composite replacement tissues. A primary strategy is an in-situ approach using a ‘cell engineered’ biofabrication process that initiates and guides tissue regeneration through a scripted usage of tools that manage the use of cells, material, chemokines, growth and morphogenic factors, including mechanical and chemical force such as fluid flow and oxygen concentration. More...

Dr. Alex Thompson, Imperial College Research Fellow, Surgical Innovation Centre, St. Mary's Hospital, Department of Medicine, Imperial College London, UK

Speech Title: A three-dimensional microscale gripper with an integrated optical force sensor for controlled micromanipulation

Abstract: Microrobotic devices have wide-ranging potential applications in biology and medicine, including targeted drug delivery, cellular manipulation and microsurgery. With the aim of developing microscale surgical robots, we have fabricated a tethered 3D microgripper with integrated force sensing on the tip of an optical fibre using two-photon polymerisation. More...

Dr. Huiyu Zhou, Reader, Department of Informatics, University of Leicester, UK

Speech Title: Mouse behavior analysis and Parkinson's disease

Abstract: Manual measurement of mouse behaviors is highly labor intensive and prone to errors. Our investigation aims to efficiently and accurately recognize individual mouse behaviors in action videos. We have built new systems inspired by the recent successful application of Fisher Vectors (FV). In the first system, each mouse action video is expressed as the collection of a set of interest points. We extract both visual and contextual features from the interest points collected from the training datasets, and then obtain two Gaussian Mixture Model (GMM) dictionaries for the visual and contextual features. The two GMM dictionaries are leveraged by FV to represent each mouse action video. A neural network is used to classify mouse action videos. In the second system, we develop and implement a novel Hidden Markov Model (HMM) algorithm to describe the temporal organisation of mouse behaviours.
Keywords: Mouse behaviour; Fisher Vector; Gaussian Mixture Model; Neural Network

Dr. Jing-Huei Lee, Professor and Graduate, Program Director, Biomedical Engineering, University of Cincinnati, USA

Speech Title: Dynamic Spatially Selective Dephasing for Magnetic Resonance Spectroscopy

Abstract: ttributing to the early invention of the slice selection technique, single-voxel magnetic resonance spectroscopy (MRS) is a widely used tool for biomedical research in vivo since the launch of the MR Imaging (MRI). The results of MRS studies often report the ratios of certain chemical compounds in tissues. More...
Keywords: MRSI, dynamic shimming, spatially selective dephasing, 13C MRS, 31P MRS

Dr. Li Zhang, Associate Professor (Reader), Department of Computer and Information Sciences, Faculty of Engineering and Environment, Northumbria University, UK

Speech Title: Intelligent Facial and Bodily Expression Recognition for a Humanoid Service Robot

Abstract: Automatic perception of human affective behaviour from facial and bodily expressions would greatly enhance natural human agent/robot interaction. This presentation will make exploration of the above research topics and focuses on adaptive intelligent facial and bodily expression recognition for a humanoid robot. Since generating an effective facial and bodily representation is a vital step to the success of emotion recognition, diverse feature extraction algorithms and evolutionary algorithm-based feature selection techniques will be explored. Other computer vision applications such as deep learning based image description generation and medical imaging will also be introduced.
Keywords: Facial and bodily expression recognition, humanoid robots, medical imaging

Dr. Perry Xiao, Associate Professor and Course Director, School of Engineering, London South Bank University, UK

Speech Title: Digital Image Processing for In-vivo Skin Capacitive Contact Imaging

Abstract: Capacitive contact imaging, originally developed for biometric applications, has shown potential in measuring skin properties including skin hydration and skin texture. Our previous studies showed it also can be used for solvent penetration measurements, skin damage assessments, as well as hair and nail water content measurements, despite the low water content. More...
Keywords: Digital image processing, capacitive contact imaging, skin, skin hydration, skin damages

Dr. Jie Yao, Professor, School of Medicine, Sun Yat-Sen University, China

Speech Title: Optical Detection of Transcriptional Activities in Single Cells

Abstract: Single cell optical imaging has become a major approach to study mechanisms of transcriptional regulation in higher eukaryotes. I will discuss our recent work in detecting transcriptional activities in cultured mammalian cells by single molecule RNA fluorescence in situ hybridization. We have successfully identified transcription sites of endogenous genes and counted nascent transcripts. We have generated single-copy promoter transgenes by site-specific DNA recombination and have quantitatively analyzed changes in promoter activities in single cells. We have studied the roles of cell cycle progression and transcription factor NF-Y binding in regulating mouse Ccnb1 gene. This work will facilitate single cell analysis on transcriptional regulation in mammals.
Keywords: Optical imaging, transcriptional regulation, mammalian cells, fluorescence in situ hybridization, single cell analysis

Dr. Sebastian Oltean, Institute of Biomedical & Clinical Sciences, University of Exeter Medical School, UK

Speech Title: Use of splicing-sensitive fluorescent reporters to screen for modulators of VEGF-A splicing in vitro and to understand splicing regulation in vivo in transgenic mice

Abstract: Through alternative splicing of the terminal exon in the VEGF-A gene, two functionally distinct isoform families are generated. Use of the canonical proximal 5’ splice site results in the expression of the pro-angiogenic VEGF-Axxx isoforms, whereas use of the distal 3’ splice site results in anti-angiogenic VEGF-Axxxb expression. More...
Keywords: alternative splicing, splicing reporters, VEGF-A

Dr. Yugen Zhang, Group Leader, Institute of Bioengineering and Nanotechnology, ASTAR, Singapore

Dr. Yawei Wang, Professor, Biomedical Optics & Imaging Technique Research Center , Jiangsu University, China.

Dr. Jun-Hee Na, Dept. of Electric, Electronic and Communication Engineering Edu., Chungnam National University, Korea

Speech Title: Application of a Contact Lens Type Artificial Iris With Self-Regulating Capability by Reversible Photoreaction

Abstract: Application of an artificial iris which provides the biocompatibility, the self-regulation capability without any peripheral component, and replaceability of iris’ function without surgery. More...

Dr. Tingting Zhu, Senior Researcher, Department of Engineering Sciences, University of Oxford, UK

Speech Title: Personalised Patient Physiology Monitoring using Bayesian Hierarchical Gaussian Processes

Abstract: With the rapid increase in volume of wearable devices, automated algorithms are employed to label physiological time-series data. The management of chronic diseases can be performed by monitoring continuous time-series vital-sign data (such as blood pressure and heart rate) via low-cost wearable devices. Automated algorithms may then be used with the resulting data to provide early warning of deterioration of the health of an individual. Such algorithms are typically trained for a large population without taking into account the time-variability and inter-subject variability of the data being collected. More...

Dr. Jang-Yeon Park, Associate Professor, MR Advanced Imaging Research Lab (AIR @ MR), Center for Neuroscience Imaging Research, Department of Biomedical Engineering, Sungkyunkwan University, Korea

Dr. Daniel S. Oh., College of Dental Medicine, Columbia University Medical Center; Founder, DSO Bone Research Institute, USA

Speech Title: Innovative Bone Regeneration in Critical Segmental Defect via Bone-like Scaffold

Abstract: An effective, long-term treatment of critical-sized bone defects remains challenging for surgeons and patients. Three key processes within the bioengineering construct, active endogenous cells recruitment into scaffold, homogenous distribution, and inhabitance together with a pro-angiogenic microenvironment is crucial for functional bone restoration. More...

Dr. Dong-Hoon Lee, Research Fellow / MRI Physicist, Faculty of Health Sciences and Brain & Mind Centre, The University of Sydney, Australia

Speech Title: Neurotransmitter Gamma-Aminobutyric Acid Mapping in Epileptic Rats Using Chemical Exchange Saturation Transfer (CEST) MRI

Abstract: The purpose of this study to demonstrate the feasibility of quantitative and qualitative mapping of Gamma-aminobutyric acid (GABA) using chemical exchange saturation transfer (CEST) technique in epileptic rats. All experimental investigations were performed as a function of time, before and after epilepsy, and the signal correlation analysis between GABA CEST and GABA concentration using 1H magnetic resonance spectroscopy (1H MRS) was also investigated. More...

More will come soon.

The 7th International Conference on Biomedical Engineering and Biotechnology
Conference Secretary General: Linda Li   Assistants: Annie Zhu, Cassie Cheng
Email: /   Tel: +86-27-87051286  Cell phone: +86-13018020541
Address: No. 1, Optical valley avenue, East Lake High-Tech Development Zone, Wuhan, Hubei, China