The 8th International Conference on Biomedical Engineering and Biotechnology (ICBEB 2019)
October 22-25, 2019, Seoul, Republic of Korea
Invited Speaker--Dr. S. Satyanarayana

Professor, Department of Chemistry, Osmania University, India

Sirasani Satyanarayana received his PhD under the supervision of Prof. M.M. Taqui Khan in 1982 at Osmania University Hyderabad, India. In 1983 he was appointed as Assistant professor at Osmania University Hyderabad, India. He worked with Prof. K.L. Brown, Mississippi State University Mississippi State, MS (USA) as Postdoctoral Associate (1989-1991). He also worked with Prof. J. B. Chaires at University of Mississippi Medical Center Jackson MS (USA) 1991-1993. He has 32 years of experience in teaching and 40 years of research experience. He guided for the award of 28 PhDs, 2 M.Phils and published 151 national and international publications in the area of Bioinorganic and Inorganic Chemistry. He worked as a Vice-Chancellor Osmania University, Hyderabad from July 2011-July 2014 and Vice-Chancellor (I/c) and Director CETLS, Rajiv Gandhi University of Knowledge Technologies (RGUKT), Basar, Telangana, from July, 2015 - August 2017.

Specialization and Research Summary:
Ph.D. in Chemistry (Bioinorganic) with four years of postdoctoral research experience in USA. Worked as Postdoctoral Associate in the Department of Chemistry, University of Texas at Arlington, Mississippi State University and University of Mississippi Medical Centre, Jackson. About ten years as leader of a group actively involved in B12 model studies.
Presently research group under him is actively involved in the synthesis of mixed ligand polypyridyl metal complexes and their interaction with DNA, Photocleavage of plasmid DNA, antimicrobial activity and anticancer activity of the complexes. The Absorbance, Fluorescence, Circular dichroism, UV melting experiments, viscosity and competition dialysis are used to study the interaction of metal complexes to DNA.

Speech Title: Investigation of DNA binding, photocleavage, molecular Docking and antitumor activity of synthesized mononuclear Ru(II) polypyridyl complexes

Abstract: A group of new mononuclear Ru(II) polypyridyl complexes designed with different ancillary ligands (phen = 1, 10 phenanthroline, bpy = 2, 2’ bipyridine, dmb = 4, 4’-dimethyl 2,2’ bipyridine) and changing intercalating ligand {CIIP = 2-(5-Chloro-3a H-Isoindol-3-yl)-1H-Imidazo[4,5-f][1,10]phenantholine}. The complexes are [Ru(phen)2CIIP]2+ (1) [Ru(bpy)2CIIP]2+ (2) and [Ru(dmb)2CIIP]2+ (3) were synthesized and characterized by various spectral methods like 1H-NMR, 13C-[H]NMR, IR, mass and electronic absorbance spectroscopy. The binding abilities of all these ruthenium complexes were investigated using absorbance, Fluorescence and viscosity experiment. The intercalate mode of binding was confirmed by viscosity experiment. All the experimental results suggested that, they can bind with DNA through intercalative mode and having different binding constant. The complexes were effectively quenched in presence of quencher and compared with different ratio of DNA. Light switch on/off experiment explains complexes act as emission sensing nature in presence of heterometal. The invitro cytotoxicity of these complexes was evaluated by MTT assay. From this it has confirmed the cytotoxicity effect of the ruthenium complex in a dose dependent manner. Annexin V FITC indicates all ruthenium complexes induce apoptosis and further supported by DNA fragmentation assay. Cell cycle analysis shows all these complexes perturbed cell cycle dynamics.

Keywords: DNA binding, Fluorescence Studies, photocleavage, antitumor studies

Fig.1. HeLa cells were treated with complexes 1, 2 and 3 at a concentration of 50 µM for 48h. After treatment, distribution of cell cycle phases was quantified by flow cytometric analysis.
The 8th International Conference on Biomedical Engineering and Biotechnology
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